Name: Anti-ARIH2/TRIAD1 antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; mouse; rat; dog; cow
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    E3 Ubiquitin-Protein Ligase Arih2; Ari-2; Protein Ariadne-2 Homolog; Ring-Type E3 Ubiquitin Transferase Arih2; Triad1 Protein (Gene ID:10425; UniProt:O95376)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IF
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:8000.
    WB-Approx 60kDa band observed in nuclear lysates of Hela and K562 Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.Customer's experiments gave a band at approx 60kDa in lysates in both Human HeLa and Mouse Muscle cells. This band disappeared after RNAi knock-down. Calculated MW of 57.8kDa according to NP_006312.1). Recommended concentration: 1-3µg/ml. This antibody has also been successfully used in WB in PMID: 24486325.
    IF-Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325.