Name: Anti-APOM antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human; dog
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Apolipoprotein M; Apo-M; Apom; Protein G3a (Gene ID:55937; UniProt:O95445)
    
    * For research use only
- Applications
  - Application
    
    ELISA; IHC
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:8000.
    WB-Preliminary experiments gave an approx 50kDa band in Human Kidney and Liver lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3kDa according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
    IHC-In paraffin embedded Human Liver shows strong stain of cytoplasm in select hepatocytes. Recommended concentration, 3-6µg/ml.