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Overview
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Recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.The alpha-tubulin (relative molecular weight about 50 kDa) is globular protein that exists in cells as partof soluble alpha/ beta-tubulin dimer or it is polymerized into microtubules. In different species it iscoded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genesare named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some havemore restricted tissue expression. Alpha-tubulin is also subject of numerous post-translationalmodifications. Tubulin isotypes and their posttranslational modifications are responsible for multipletubulin charge variants-tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminalstructural domain.Immunogen: Fraction of tubulin purified from porcine brain by two cycles of polymerization-depolymerization.
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Overview