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Overview
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Akt1 is a protein encoded by the oncogene AKT1 which is approximately 55, 7 kDa. Akt1 is localised to the cytoplasma, nucleus and cell membrane. It is involved in RET signalling, the regulation of lipid metabolism, insulin signalling-generic cascades, common cytokine receptor gamma-chain family signalling pathways and apoptotic pathways. This protein falls under the AKT kinase family. The AKT1 isoform has a specific role in cell motility and proliferation. By activating serine/threonine kinase AKT1 apoptosis can be suppressed by phosphorylating and therby inactivating components of the apoptotic machinery. Mutations in the AKT1 gene can result in breast cancer, colorectal cancer, Cowden syndrome 6 and Proteus syndrome which causes overgrowth. STJ91542 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Akt1 protein only when phosphorylated at T450.
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Overview
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Akt Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HepG2 cells, treated with Serum 30% 30', using Akt Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of various cells using Akt1 Polyclonal Antibody diluted at 1：2000 Immunofluorescence analysis of HeLa cells, using Akt Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, Akt1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1, Akt1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-spleen tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Akt1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-spleen tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of human-stomach tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of human-stomach tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of human-lung tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of human-lung tissue. 1, Akt1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Anti-Akt1 antibody
Anti-Akt1 antibody
Catalog Number:
A001046913STJ
Mfr. No.:
STJ91542
Price:
$224
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