Name: Anti-AIRE antibody
Price: 499.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Autoimmune Regulator; Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein; Apeced Protein (Gene ID:326; UniProt:O43918)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; IHC; IF; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:128000.
    WB-Preliminary testing showed an approx 60kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates. Recommended concentration: 1-2µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Smooth Muscle lysate. This product has been successfully used in Western blot by an anonymous customer on HEK293 cell lysate.
    IHC-In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5µg/ml.
    IF-Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10µg/ml.
    FC-Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.