Amino-dPEG®₈-t-boc-hydrazide

Amino-dPEG®8-t-boc-hydrazide, product number 10957, is a monodisperse PEGylation reagent designed to crosslink carboxylates with aldehydes or ketones. The ends of the amphiphilic dPEG® spacer terminate with a free amine and a boc-protected hydrazide group. After the amino group reacts with a carboxylate or active ester (NHS or TFP), the tert-butyl protecting group deprotects with acid. The exposed hydrazide moiety then reacts with aldehydes or ketones, forming a hydrazone bond.

Why use dPEG® products?
Many conventional crosslinkers are hydrophobic. Hydrophobic crosslinkers cause well-known problems with biomolecules in aqueous media. These problems include aggregation, precipitation, and non-specific interactions. Consequently, among other issues, hydrophobic crosslinkers cause signal attenuation, signal loss, and false signaling (positive or negative).

One solution to the problem of hydrophobic crosslinkers is to insert a polyethylene glycol (PEG) chain between the crosslinker functional groups. PEG is amphiphilic (meaning that it dissolves in both water and organic solvents). Consequently, PEG increases water solubility and adds hydrodynamic volume to conjugates made with it. However, traditional PEGylation reagents are non-uniform (disperse) polymers. PEG dispersity creates analytical complexity for any conjugate made with conventional PEGylation reagents.

OurdPEG® products are different. Because they are synthesized from high purity starting materials, our dPEG® products are monodispersed PEGylation reagents. Each dPEG® product contains a discrete chain length of PEG with a single molecular weight rather than a mixture of different chain lengths and molecular weights of PEG. The dPEG® difference includes a simplified analysis of conjugates, which saves our customers time and money.

Please contact us at for specific academic pricing.