AMD1 Recombinant Protein

AMD1 Recombinant Protein

Catalog Number:
P001417049ABE
Mfr. No.:
Abe32-2151
Price:
  • Size:
    5 µg
    Quantity:
    Add to Cart:
      • Overview
        • AMD1 Human Recombinant produced in E. coli is a single polypeptide chain containing 292 amino acids (68-334) and having a molecular mass of 33.4 kDa. AMD1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Adenosylmethionine decarboxylase proenzyme (AMD1) is synthesized originally as an inactive proenzyme. Putrescine stimulates both the proenzyme processing and the catalytic activity. The catalytic activity is inhibited by iodoacetic acid. The active enzyme formation entails a self-maturation process in which the active site pyruvoyl group is produced from an internal serine residue using an autocatalytic post-translational modification.

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      • Properties
        • Protein Name
          Adenosylmethionine decarboxylase 1, S-adenosylmethionine decarboxylase proenzyme, AdoMetDC, S-adenosylmethionine decarboxylase 1, SAMDC, AMD, EC 4.1.1.50.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 80% as determined by SDS-PAGE.
          Formulation
          The AMD1 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 20% glycerol.
          Storage
          Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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