Afla B Immunoaffinity Column

Using monoclonal antibody-based affinity chromatography, Afla B is a quantitative method that can isolate aflatoxins B1 and B2 from feeds, food, grains, and nuts via fluorometric or HPLC detection. Afla B is sensitive, simple, and fast, enabling quick tests for parts per billion or parts per trillion levels. Afla B can be used in a wide variety of locations from the local farm elevator to food QC and safety laboratories.

Benefits
• Convenient — For use with fluorometer or HPLC
• Durable — Long shelf life; requires no refrigeration
• Versatile — For use with a variety of samples
• Quick — 10 minutes to isolate toxin*
• Ideal Volume- Column size of 1 mL
• Wide Range — Detects levels as low as 1 ppb (fluorometer) and 50 ppt (HPLC) and as high as 300 ppb
• Safe — Requires less toxic materials than other methods
*excludes preparation and extraction

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Background

Aflatoxin, a toxin from a naturally occurring mold, is a Group 1 carcinogen proven to cause cancer in humans. The United States federal government action level for aflatoxin in food for human consumption or in dairy cowfeed is 20 ppb. For milk for human consumption the level is 0.5 ppb. Levels up to 300 ppb are allowed in certain circumstances for nondairy animal feed. The European Union’s aflatoxin regulations for groundnuts, tree nuts, dried fruit, spices, and cereals include limits as low as 4 ppb total aflatoxins and 2 ppb for aflatoxin B1. Some EU member states have also separately established their own maximum limits on aflatoxins.