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Figure 1. Immunohistochemical identification of Growth Hormone-Releasing Hormone Receptor in breast carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GHRHR (Growth Hormone-Releasing Hormone Receptor) antibody (7TM0247N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, GHRH receptors were uniformly detected at the plasma membrane in breast carcinoma.

Figure 2. Immunohistochemical identification of Growth Hormone-Releasing Hormone Receptor in ovarian carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GHRHR (Growth Hormone-Releasing Hormone Receptor) antibody (77TM0247N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, GHRH receptors were detected predominantly at the level of plasma membrane in ovarian carcinoma.

Figure 3. Immunohistochemical identification of Growth Hormone-Releasing Hormone Receptor in prostate carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GHRHR (Growth Hormone-Releasing Hormone Receptor) antibody (77TM0247N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, GHRH receptors were detected predominantly at the level of plasma membrane in prostate carcinoma.

Figure 4. Validation of the Growth Hormone-Releasing Hormone Receptor in pituitary adenoma. Growth hormone-producing pituitary adenoma was homogenated and immunoblotted using anti-GHRHR (Growth Hormone-Releasing Hormone Receptor) antibody (7TM0247N-IC) at a dilution of 1:1000 in presence (+) or absence (-) of 10 µg/ml peptide antigen. Note, absence of peptide leads to detection of GHRH receptors in pituitary adenoma but not in presence of peptide.

GHRHR ( IHC-grade ), GHRH Receptor Polyclonal Antibody

GHRHR ( IHC-grade ), GHRH Receptor Polyclonal Antibody

Catalog Number:

A001455995TMA

Mfr. No.:

7TM0247N-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The GHRH receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Growth Hormone-Releasing Hormone Receptor. It detects the canonical form of GHRHR as well as the amino-terminal splice variant SV1. It can be used to detect total GHRH receptors in Western blots independent of phosphorylation. The GHRHR antibody can also be used to isolate and enrich GHRH receptors from cell and tissue lysates. It also detects GHRHR in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide presents distal end of carboxyl-terminal tail of human GHRHR
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  GHRHR, Growth Hormone Releasing Hormone Receptor (UniProt:Q02643)
  
  * This product is intended for research use only.

Applications

Application

WB; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100

Reference
- Schulz S, Röcken C, Schulz S. Immunocytochemical localisation of plasma membrane GHRH receptors in human tumours using a novel anti-peptide antibody. Eur J Cancer. 2006 Sep;42(14):2390-6. Epub 2006 Aug 14. PubMed PMID: 16904887.

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