Acid-dPEG®₂₅-NHS ester

Acid-dPEG®25-NHS ester, product number 10140, is a novel, amine-reactive crosslinker from Quanta BioDesign, Ltd. This product is designed to facilitate intermolecular crosslinking with free amines at each end of the crosslinker through a single molecular weight, discrete polyethylene glycol (dPEG®) backbone. The correct use of this product eliminates intramolecular crosslinking. The dPEG® backbone is amphiphilic. Consequently, it imparts water solubility to molecules to which it is conjugated. One end of the chain terminates in a propionic acid group. The other end terminates as the N-hydroxysuccinimidyl (NHS) ester of propionic acid.

Traditional Homobifunctional Crosslinkers
Homobifunctional crosslinkers join two different molecules using the same reactive group. Traditional homobifunctional crosslinkers are hydrophobic. With hydrophobic crosslinkers, aggregation and precipitation of conjugated molecules can be a severe problem. Moreover, all homobifunctional crosslinkers tend to polymerize uncontrollably. Also, when conjugating large biomolecules such as proteins, homobifunctional crosslinkers react both intermolecularly and intramolecularly. Such mixed reactions create a wide variety of products that are difficult to characterize.

The dPEG® difference with Acid-dPEG®25-NHS ester
Quanta BioDesign designed Acid-dPEG®25-NHS ester, product number 10140, to overcome the problems that typically accompany the use of homobifunctional crosslinkers.

Firstly, the amphiphilic dPEG® backbone imparts water solubility to conjugates. Our dPEG® technology creates unique PEG-based products that are soluble in both organic and aqueous media. These products provide all the benefits of traditional PEG products without the attendant difficulties of dispersity.

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