AccuCount Rainbow Particles 7.0-7.9 um

Shake bottle vigorously or vortex briefly before use.

Add the monoclonal antibody of your choice to 100 μL of test sample. Incubate, lyse, wash, and then resuspend in 1 to 2 mL of PBS. If staining and lysing are not needed, just mix a known volume of test sample in 1 to 2 mL of PBS.
Add exactly 50 μL of ACFP-70-10 particles to the suspension. Precision when pipetting is absolutely critical.
Run the sample in the flow cytometer and obtain the amount of events for the ACFP-70-10 particles and your test sample.
Calculate the number of cells accordingly.

DO NOT WASH ACCUCOUNT PARTICLES WITH THE SAMPLE PRIOR TO ANALYSIS.FOR RESEARCH APPLICATIONS ONLY. NOT FOR DIAGNOSTIC USE.

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