Name: AAVrh.74 Reference (Byproduct-Empty)
Price: 558.00 USD

- Overview
  - AAVrh.74 Reference (Byproduct-Empty) particles are byproducts of regular AAVrh.74-CMV-EGFP-2.8kb vectors produced with our pAAVone-AAVrh.74-CMV-EGFP-2.8kb plasmid. Unlike AAV truly-empty particles, AAV byproduct-empty particles may contain partial or incomplete genomes [Wang et al., 2016]. They are not completely devoid of genetic material, and this genetic content can vary in terms of completeness. Byproduct-empty AAV particles are not the primary focus of production and can sometimes be considered impurities or contaminants in the final AAV vector preparation.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    AAV references (Full) and AAV references (Byproduct-Empty) are generated using the AAVone system, with the corresponding pAAVone plasmids carrying a 2.8 kb AAV-CMV-SV40I-EGFP-WRRE-hGHpolyA genome. AAV references (Truly-Empty) are produced by transfection of a single mini-pHelper-AAV plasmid with the corresponding serotypes. AAV reference materials are available for various serotypes, including AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, AAVrh.10, and AAVrh.74. AAV references with other serotypes and genomes are available upon request.
- Properties
  - Other Properties
    
    AAV Serotype: AAVrh.74
    AAV Capsid titer: 1.0×10^13 VP/ml (VP: Viral Particle) by Elisa
    Used cell line: HEK 293T
    Used plasmid: pAAVone-AAVrh.74-CMV-EGFP-2.8kb
    AAV Genome: AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA-2.8kb
    Production system: AAVone® [Yang et al., 2025]
    Purification: AAVx followed by one round of CsCl ultracentrifuge.
    Buffer: 0.001% F-68/DPBS with additional 150mM NaCl.
    Endotoxin: <1U/1.0×10^13 VP
    
    Storage
    
    -80°C for long term (> 1 year); -20°C for short term (1-2 months); 4°C for 1-2 weeks..
    
    Shipping
    
    Dry ice.
- Applications
  - Application Description
    
    Empty AAV particles can serve as valuable tools for various stages of gene therapy development and characterization.
    · Reference Materials: Well-characterized empty AAV capsids, can serve as standardized reference materials for analytical assays.
    · Quality Control: During the manufacturing process of AAV vectors, using well-characterized capsids as reference materials allows manufacturers to monitor and ensure the quality, purity, and consistency of their AAV products.
    · Biodistribution Studies: Empty AAV can be used in biodistribution studies to assess how different capsids distribute in various tissues after administration.
    · Immunogenicity Assessment: By exposing the immune system to well-characterized AAV capsids, researchers can evaluate the immune responses induced by specific serotypes.
    · Comparative Studies: Empty AAV allow researchers to compare the characteristics of different AAV serotypes without the confounding effects of transgene expression.
    · Process Development: Empty AAV can be used in the development and optimization of manufacturing processes for AAV vectors.
    · Assay Development: Empty AAV capsids serve as positive controls in various assays, aiding the development of new methods for AAV characterization, stability testing, and potency determination.
    · Gene Therapy Capsid Decoys: The presence of an excess of empty AAV capsids may effectively absorb low-level neutralizing antibodies (NAbs) and non-NAbs, permitting transduction even in their presence.
- Reference
  - Syngeneic AAV Pseudo-particles Potentiate Gene Transduction of AAV Vectors
    AAVone: A Cost-Effective, Single-Plasmid Solution for Efficient AAV Production with Reduced DNA Impurities

AAVrh.74 Reference (Byproduct-Empty)

AAVrh.74 Reference (Byproduct-Empty)

Background

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