AAV6 Reference-4.3kb

The AAV6 reference material contains a 4.3kb AAV-CMV-SV40I-EGFP-WPRE-hGHpolyA genome (with a 1.5kb LacZ) in AAV6 capsid, with a genome titer (EGFP targeting) of 1X10^13 VG/ml. This reference is produced using the AAVone system with the pAAVone-AAV6-CMV-EGFP-4.3kb plasmid. AAV6 is a naturally occurring serotype of adeno-associated virus that has gained attention for its ability to transduce a wide range of tissues, particularly muscle, lung, and liver. AAV6 shares some similarities with AAV1, as both belong to the same clade, but AAV6 has unique properties that make it a preferred choice for certain gene therapy applications, especially those targeting muscle and the respiratory system. AAV6 is widely used in T cell engineering, particularly in the field of chimeric antigen receptor (CAR) T cell therapy.

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Background

AAV references are crucial for qPCR-based vector genome quantification and fundamental for ensuring accuracy in viral vector research and gene therapy development. Our AAV reference materials undergo extensive characterization, including full ratio (mass photometry), genome titer (ddPCR), capsid titer (ELISA), purity (SDS-PAGE silver staining), and tests for bioburden, endotoxin, and mycoplasma. Quantification is based on reference material from ATCC. These thoroughly analyzed particles provide precise genome titers, well-defined full-to-empty capsid ratios, and accurate capsid titers. This comprehensive characterization is essential for standardizing measurements, ensuring consistency across experiments, and delivering reliable data for gene therapy applications and other research involving AAV vectors.
AAV references (Truly-Empty) are produced by transfection of a single mini-pHelper-AAV plasmid with the corresponding serotypes. AAV references (Full) and AAV references (Byproduct-Empty) are generated using the AAVone system, with the corresponding pAAVone plasmids carrying AAV-CMV-SV40I-EGFP-WRRE-hGHpolyA genomes.