AAV-Tie1-EGFP

AAV-Tie1-EGFP series viruses are ready to use AAV vectors carrying Tie1-EGFP expression cassette. The AAV-Tie1-EGFP vectors are available with 16 different serotypes, and there are over 1000 AAV serotypes/variants to be choose for custom AAV packaging.
The Tie1 promoter is a regulatory sequence that controls the expression of the Tie1 (Tyrosine kinase with immunoglobulin-like and EGF-like domains 1) gene. Tie1 is a receptor tyrosine kinase that plays a critical role in the development and maintenance of the vascular system, particularly in endothelial cells, which line the interior surface of blood vessels. The Tie1 promoter is an endothelial cell-specific regulatory element that drives gene expression in the vascular endothelium. It is a powerful tool in vascular research, gene therapy, and the development of transgenic models for studying blood vessel formation, endothelial function, and related diseases. It is specificity to endothelial cells makes it invaluable for targeting gene expression within the vascular system, particularly in the context of angiogenesis and vascular disorders.
By employing EGFP as a reporter, researchers gain the ability to effortlessly visualize and quantify the transduction efficiency of AAV vectors within target cells, further enhancing the precision of their experiments.

Please contact us at for specific academic pricing.

Background

AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.

To ensure high-quality and pure products, various assays are used to assess the quality and purity of their AAV vectors:
· Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
· Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
· Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
· SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
· Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.