AAV Serotype Testing Kit-CMV-mCherry

The AAV Serotype Testing Kit, featuring the CMV-mCherry system, enables a systematic evaluation of 16 distinct AAV serotypes for gene delivery across diverse cell types and tissues. In this kit, you'll find a collection of 16 AAV serotypes, each possessing unique capsid proteins. These serotypes exhibit variations in their capacity to transduce various cell types and tissues, rendering them valuable tools for refining gene therapy and delivery experiments.
The CMV promoter serves as the driving force behind the expression of the mCherry reporter gene within these AAV vectors. The CMV promoter, also known as the cytomegalovirus promoter, is a widely used promoter sequence in molecular biology and biotechnology. It is derived from the immediate early promoter of the cytomegalovirus and is commonly used to drive the expression of genes in a variety of experimental and practical applications.
By employing mCherry as a reporter, researchers gain the ability to effortlessly visualize and quantify the transduction efficiency of AAV vectors within target cells, further enhancing the precision of their experiments.

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Background

In this category, 15 most common serotypes of AAV vectors (AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAVrh.10, AAVrh.74, AAV2-Retro) are included in each kit. The vectors carry fluorescent protein (EGFP, mCherry, Tdtomato) or reporter luciferase, which is driven by promoter CAG, CMV, EF1a or hSYN. The kits are pretended to provide useful tools for customers, with minimum cost, in testing AAV transduction efficiency, testing tissue specific tropism related to serotypes, and using as negative control to examine whether the observed biological effects come from specific transgenes. AAV serotype testing kits can be purchased directly as they are, or they can be customized based on the specific needs of the customer.These kits are designed to quickly and efficiently identify a limited set of commonly used AAV serotypes that are suitable for specific cell lines or tissues. This method is likely useful when you have a clear understanding of the cell lines or tissues you intend to target and want a simple way to narrow down your choices to a smaller subset of AAV serotypes. The testing process probably involves exposing the cells or tissues to various AAV serotypes and observing how effectively they transduce the target cells. This can provide you with a preliminary indication of which AAV serotypes are most effective for your specific experimental setup.