AAV-EF1a-Gluc

AAV-EF1a-Gluc series viruses are ready to use AAV vectors carrying EF1a-Gluc expression cassette. The AAV-EF1a-Gluc vectors are available with 16 different serotypes, and there are over 1000 AAV serotypes/variants to be choose for custom AAV packaging.
The EF1a promoter refers to the promoter region of the Elongation Factor 1-alpha (EF1a) gene, which is responsible for driving the expression of EF1a, a protein that plays a key role in the elongation phase of protein synthesis. The EF1a promoter is a strong, ubiquitous promoter that drives constitutive gene expression across a wide range of cell types. It is widely used in gene therapy, transgenic model creation, protein production, and research on gene function. Its broad activity and ability to drive high-level expression make it a valuable tool for experiments requiring robust and consistent gene expression in diverse biological systems.
Gaussia luciferase (Gluc) is a highly sensitive bioluminescent reporter protein commonly used in biological research to monitor gene expression, track cellular processes, or assess promoter activity in real time.
By employing Gluc as a reporter, researchers gain the ability to effortlessly quantify the transduction efficiency of AAV vectors within target cells.

Please contact us at for specific academic pricing.

Background

AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.

To ensure high-quality and pure products, various assays are used to assess the quality and purity of their AAV vectors:
· Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
· Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
· Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
· SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
· Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.