AAV-Col2a1-EGFP

AAV-Col2a1-EGFP series viruses are ready to use AAV vectors carrying Col2a1-EGFP expression cassette. The AAV-Col2a1-EGFP vectors are available with 16 different serotypes, and there are over 1000 AAV serotypes/variants to be choose for custom AAV packaging.
The Col2a1 promoter refers to the regulatory sequence that controls the expression of the Collagen Type II Alpha 1 (Col2a1) gene. The Col2a1 gene encodes type II collagen, which is a major component of cartilage, making this promoter particularly important in the context of chondrocyte biology and cartilage-related research. The Col2a1 promoter is highly specific to chondrocytes, the cells responsible for producing and maintaining the cartilaginous matrix in joints and other cartilaginous tissues. This specificity makes it a crucial tool for targeting gene expression in cartilage.
By employing EGFP as a reporter, researchers gain the ability to effortlessly visualize and quantify the transduction efficiency of AAV vectors within target cells, further enhancing the precision of their experiments.

Please contact us at for specific academic pricing.

Background

AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.

To ensure high-quality and pure products, various assays are used to assess the quality and purity of their AAV vectors:
· Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
· Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
· Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
· SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
· Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.