AAV-CMV-BFP

AAV-CMV-BFP series viruses are ready to use AAV vectors carrying CMV-BFP expression cassette. The AAV-CMV-BFP vectors are available with 16 different serotypes, and there are over 1000 AAV serotypes/variants to be choose for custom AAV packaging.
The CMV promoter, also known as the cytomegalovirus promoter, is a widely used promoter sequence in molecular biology and biotechnology. It is derived from the immediate early promoter of the cytomegalovirus and is commonly used to drive the expression of genes in a variety of experimental and practical applications. BFP is a fluorescent marker used in biological research to label cells, visualize gene expression, and track cellular processes. Its blue fluorescence provides a distinct signal that can be easily differentiated from other fluorescent proteins, making it useful in multi-color labeling experiments. AAV-BFP provides researchers with a powerful tool for visualizing and analyzing cellular processes in a straightforward and reliable manner, especially in experiments requiring multiple fluorescent markers.

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Background

AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.

To ensure high-quality and pure products, various assays are used to assess the quality and purity of their AAV vectors:
· Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
· Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
· Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
· SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
· Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.