AAV-CaMKIIα(0.36)-Rluc

AAV-CaMKIIα(0.36)-Rluc series viruses are ready to use AAV vectors carrying CaMKIIα(0.36)-Rluc expression cassette. The AAV-CaMKIIα(0.36)-Rluc vectors are available with 16 different serotypes, and there are over 1000 AAV serotypes/variants to be choose for custom AAV packaging.
The 0.36kb CaMKIIα promoter refers to a shortened version of the promoter region for the Calcium/Calmodulin-Dependent Protein Kinase II Alpha (CaMKIIα) gene. CaMKIIα is a crucial enzyme in the brain, particularly in neurons, where it plays a significant role in synaptic plasticity, learning, and memory. This 0.36kb version of the promoter is specifically designed to drive gene expression in neurons, particularly in excitatory neurons of the forebrain, such as those in the hippocampus and cortex.
Renilla luciferase (Rluc) is a bioluminescent reporter protein derived from the sea pansy Renilla reniformis. Rluc catalyzes the oxidation of its substrate, coelenterazine, to produce light, typically in the blue to green range. This property makes Rluc a widely used tool in biological research, particularly for real-time imaging and monitoring of cellular processes. AAV-Rluc is a valuable tool for researchers who need to perform sensitive and non-invasive imaging and monitoring of gene expression and biological processes. Its high sensitivity, low background, and versatility make it particularly useful for in vivo studies, dual-reporter assays, and real-time tracking of cellular events.

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Background

AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.

To ensure high-quality and pure products, various assays are used to assess the quality and purity of their AAV vectors:
· Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
· Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
· Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
· SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
· Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.