AAV-CAG-BFP

AAV-CAG-BFP series viruses are ready to use AAV vectors carrying CAG-BFP expression cassette, in which the BFP reporter is droved by a CAG promoter. The AAV-CAG-BFP vectors are available with 16 different serotypes, and there are over 1000 AAV serotypes/variants to be choose for custom AAV packaging.
The CAG promoter is a synthetic, hybrid and ubiquitous promoter widely used in molecular biology to drive high levels of gene expression in mammalian cells. It is particularly valued for its strength and broad activity across various cell types, making it a popular choice for applications such as gene therapy, transgenic animal models, and research studies that require robust and consistent gene expression. It is composed of three key elements:

· CMV Immediate-Early Enhancer: Derived from the cytomegalovirus, this enhancer sequence provides strong and ubiquitous transcriptional activation in a variety of cell types.
· Chick Beta-Actin Promoter: The core promoter region is derived from the chicken beta-actin gene, which is known for its high expression levels in many cell types, particularly in the context of gene expression in vertebrates.
· Rabbit Beta-Globin Intron: An intronic sequence from the rabbit beta-globin gene is included to enhance transcriptional efficiency and mRNA stability, further boosting expression levels.

The BFP reporter used in this AAV vectors is mTagBFP2, a basic (constitutively fluorescent) blue fluorescent protein published in 2011, derived from Entacmaea quadricolor. It is reported to be a very rapidly-maturing monomer with very low acid sensitivity. Its blue fluorescence provides a distinct signal that can be easily differentiated from other fluorescent proteins, making it useful in multi-color labeling experiments. AAV-BFP provides researchers with a powerful tool for visualizing and analyzing cellular processes in a straightforward and reliable manner, especially in experiments requiring multiple fluorescent markers.

Please contact us at for specific academic pricing.

Background

AAV viruses or AAV vectors, are a convenient solution for researchers who need to perform gene delivery experiments, such as gene therapy, gene editing, and gene regulation. The premade AAV viruses are available in various serotypes, promoters, and reporters/transgenes, allowing researchers to choose the most suitable vector for their specific research application. With over a thousand known AAV serotypes, customers have the tools to rapidly determine the vector specificity for various cell types. Additionally, the availability of multiple promoters paired with reporter genes allows for easy assessment and monitoring of gene expression levels and timing.

To ensure high-quality and pure products, various assays are used to assess the quality and purity of their AAV vectors:
· Gene specific qPCR Titer: Target the specific transgenes or common used elements, such as WPRE, CMV, PolyA, but not ITR.
· Full/Empty Particle Ratio: Ensure all the premade AAVs has >80% full particles by Mass Photometry.
· Endotoxin Testing: Ensure safety with endotoxin level <1U/1e13VG.
· SDS-PAGE Gel: Assess the purity of the AAV viruses is great than 95%.
· Infectivity Assay: Evaluate the efficiency of AAV viruses in transducing target cells if they have reporters.