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Coomassie-stained SDS-PAGE. Lane 1: Molecular weight markers; Lane 2: 5 µg purified 6xHis-PA28beta

Activation of 5 nM 20S proteasome (Cat. # A1400) by 25 nM PA28β (Cat. # A2200), the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC (Cat. # G1100) as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

6xHis-PA28beta

6xHis-PA28beta

Catalog Number:

PAS1097538UBP

Mfr. No.:

A2200

Price:

$280

Size:

25 µg

Quantity:

Add to Cart:

- Overview
  - PA28 (also called the 11S regulatory complex) is another activator of the 20S proteasome, which assembles on either one or both ends of the 20S proteasome in an ATP-independent manner. PA28 can greatly enhance the peptidase activities of the 20S proteasome, but not for degradation of ubiquitinated proteins. The PA28 activator is a complex of two alternating homologous subunits, PA28α and PA28β, which assembles as a hexameric ring with an α3β3 stoichiometry. PA28α and PA28β are induced by interferon. Functionally, PA28 enhances the generation of class I peptides for antigen presentation. The third subunit, PA28γ, was recently identified in the nucleus. Recombinant PA28α and PA28β form a heptomeric ring; whereas PA28γ forms a hexameric ring. All of them can stimulate the peptidase activities of the 20S proteasome.
    
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- Properties
  - Protein Name
    
    PSME2;PA28B; REGbeta; PA28beta;PA28-beta;PA28 beta
    
    Source
    
    Bacterial recombinant
    
    Formulation
    
    20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
    
    Storage
    
    Store at -80°C; avoid multiple freeze-thaw cycles
    
    Concentration
    
    See tube label
    
    Molecular Weight
    
    27.4 kDa
    
    Sequence
    
    NCBI RefSeq: NM_002818
    
    * Research Use Only

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