Name: 6-Carboxyfluorescein-N-hydroxysuccinimide ester
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- Overview
  - 6-Carboxyfluorescein is a fluorescent dye widely used in PCR methodologies. It is used to label the 5’ end of oligonucleotides as a reporter in the presence of a quencher at the 3’ end (dual labelled probe). During amplification, the dye is cleaved, and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. It is used to tag DNA and RNA for in vivo and in vitro applications, as well as for structural studies and RNA-protein interactions. 6-Carboxyfluorescein that has NHS-activated carboxylic acids can react with primary amines. 5’-reporter used in RT-PCR for COVID-19 diagnosis by HKI; Institute Pasteur; Charité; CDC, USA; CDC, China; NIID and NIH. (ex/em: 495/517nm in DMSO or DMF at pH> 9.0)
    
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- Properties
  - Categories
    
    Fine Chemicals
    
    Alternative Name
    
    6-Carboxyfluorescein-NHS;6-Carboxyfluorescein-N-succinimidyl ester;1-[[3,6-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9-[9H]xanthen ]-6-yl]carbonyl]oxy]-2,5-pyrrolidinedione
    
    CAS Number
    
    92557-81-8
    
    Molecular Formula
    
    C25H15NO9
    
    Molecular Weight
    
    473.39 g/mol
    
    MDL Number
    
    MFCD00077323
    
    Note
    
    There is no hazardous surcharge associated with this product.
    There is no special packaging charge associated with this product.
    
    * For research use only. Not for human or veterinary use
- Reference
  - Adamczyk., et al. (1997). Preparation of succinimidyl and pentafluorophenyl active esters of 5- and 6-carboxyfluorescein. Bioconjugate Chemistry 8: 253-255
    Livak., et al. (1995). Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods and Applications 4(6): 357-362
    Zhao., et al. (2018). In vivo fluorescence imaging of hepatocellular carcinoma using a novel GPC3-specific aptamer probe. Quantitative Imaging in Medicine and Surgery 8(2): 151-160