Name: 5-Carboxyfluorescein-N-hydroxysuccinimide ester
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- Overview
  - 5-Carboxyfluorescein(5-FAM) is a self-quenching dye and one of the most common labelling agents for biomolecules, cell staining and localisation studies. The fluorescence is pH dependent, therefore it has been used to measure the internal pH of cells. As it is only membrane permeable to dead cells, it can be used as a staining agent to distinguish viable and non-viable cells. It is also used in PCR to label the 5’ end of oligonucleotides in the presence of a quencher at the 3’ end (dual labelled probe). During the amplification process, the dye is cleaved and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. The carboxylic acid can be activated to react with a primary amine.
    
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- Properties
  - Categories
    
    Fine Chemicals
    
    Alternative Name
    
    5-Carboxyfluorescein-NHS;5-Carboxyfluorescein-N-succinimidyl ester
    
    CAS Number
    
    92557-80-7
    
    Molecular Formula
    
    C25H15NO9
    
    Molecular Weight
    
    473.39 g/mol
    
    MDL Number
    
    MFCD00077322
    
    Note
    
    There is no hazardous surcharge associated with this product.
    There is no special packaging charge associated with this product.
    
    * For research use only. Not for human or veterinary use
- Reference
  - Brinkley., et al. (1992). A Brief Survey of Methods for Preparing Protein Conjugates with Dyes, Haptens, and Cross-Linking Reagents. Bioconjugate chemistry 3:2-13
    Nanda., et al. (2014). Labelling a Protein with fluorophores using NHS ester derivatization. Method in Enzymology. Book Vol 536 chapter 8:87-94
    Rao., et al. (1986). Intracellular fluorescent staining with carboxyfluorescein: a rapid and reliable method for quantifying dye-coupling in mammalian central nervous system. Journal of neuroscience methods 16:251-263