Name: 5-BrdU
Price: 188.00 USD

- Overview
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    Background
    
    5-Bromodeoxyuridine (5-BrdU) is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by monoclonal antibodies or polyclona111.
    In vitro: The effect of 5-BrdU on the proliferation was studied in the early phases of dedifferentiation of Nicotiana glauca pith explants grown. It was found to be wholly inhibitory only when given during the first 72 h of culture, this inhibition being reversed by simultaneous addition of either deoxyeytidine or thymidine [1].
    In vivo: Young adult offspring mice were stained for 5-BrdU using a monoclonal antibody and the peroxidase-antiperoxidase method. The distribution of immunoreactive nuclei was very similar to the previous [3H] thymidine data. The pattern of labelled nuclei at different embryonic ages followed the spatiotemporal gradients described for cortical and hippocampal neurogenesis. These data indicate that BrdU can be used to map neuronal birthdates [2].
    Clinical trials: Currenlty no clinical data are available.
    
    [1] M. Durante, C. Geri, V. Nuti-Ronchi, G. Martini, E. Guillé, J. Grisvard, L. Giorgi, R. Parenti, M. Bulatti. Inhibition of Nicotiana glauca pich tissue proliferation through incorporation of 5-BrdU into SNA. Cell Differentiation, Volume 6, Issue 1, June 1977, Pages 53-63
    [2] del Rio JA, Soriano E. Immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. Brain Res Dev Brain Res. 1989 Oct 1; 49 (2): 311-7.
- Properties
  - Categories
    
    Synthetic thymidine analog
    
    Alternative Name
    
    5-bromo-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione
    
    CAS Number
    
    59-14-3
    
    Molecular Formula
    
    C9H11BrN2O5
    
    Molecular Weight
    
    307.1
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    insoluble in EtOH; ≥11.56 mg/mL in 0.9% NS with ultrasonic; ≥15.35 mg/mL in DMSO; ≥16.23 mg/mL in H2O with ultrasonic
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    BrC(C(N1)=O)=CN(C1=O)[[email protected]@]2([H])C[[email protected]@](O)([H])[[email protected]@](O2)([H])CO
    
    * For Research Use Only
- Reference
  - 1. Julian Shen, Wei Wei, et al. "Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK1/2 and miR-17~92a Cluster Activation." Evid Based Complement Alternat Med 2020 Aug 22;2020:7275246. PMID:32908568