384 well plates Poly-D-lysine coated

Clear, white and black solid Poly-D-lysine Coated 384 Well Plates are manufactured in pure polystyrene with low fluorescence and can be used for ELISA assays and for animal and plant cell tissue-culture studies.

Black Transparent Bottom and White Transparent Bottom Poly-D-lysine Coated 384 Well Plates combine the advantageous optical properties of virgin crystalline polystyrene with the versatility of a microplate. These transparent bottom plates offer high optical quality and low autofluorescence to suit a wide range of samples for accurate and reliable light and fluorescence readings.

White and black 384 well plates have a minimal light scatter and prevent well-to-well crosstalk. All Black 384 Well Plates have minimum back-scattered light and background fluorescence: they are recommended for florescence measurements. All White 384 Well Plates have maximum reflection and minimal autoluminescence: they are recommended for luminescence measurements.

The 384 well plates allow sample volume reduction and improve screening effeciency.

The Poly-D-lysine Coated 384 Well Plates comply with SBS standards and the design assures a good performance in automatic processing plant.

Surface:
Poly-D-lysine Coated 384 Well Plates have a positively charged surface; this features has been shown to enhance cell attachment, growth and differentiation of some cell types (e.g. primary neurons, glial cells, neuroblastomas and a variety of transfected cell lines). Many cell types adhere better to this surface and are less dependent on the presence of serum proteins. In addition, Poly-D-Lysine coated surfaces are often used to reduce cell detachment that often occurs during multiple washing steps that are associated with cell-based assays.
Every single unit contains 5 items.

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Background

Poly-D (or L)-Lysine coated surface promotes attachment and growth of various cell types under serum free and/or low serum conditions.

Poly-lysine is a synthetic positively charged polymer, existing as two enantiomers, poly-D-lysine (PDL) and poly-L-lysine (PLL). Both are commonly used however poly-D-lysine is not degraded by cellular proteases and is therefore often the preferred choice. As poly-D (or L)-lysine is a synthetic protein, it does not influence the signaling pathways of the cells and is completely free of any animal contaminants.

Poly-D (or L)-Lysine is useful in promoting cell adhesion to solid substrates and enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface.

When adsorbed to the polystyrene surface, poly-D (or L)-lysine increases the number of positively-charged sites available for cell binding. This uniform net positive charge is preferred by certain cell types and can subsequently enhance cell attachment, growth and differentiation of these cell types, especially under serum-free and/or low serum conditions.

Poly-D (or L)-lysine coated surfaces often improve attachment and growth of primary neurons, glial cells, neuroblastomas, and a variety of transfected cell lines. In addition, these coated surfaces are often used to reduce cell detachment that often occurs during multiple washing steps that are associated with cell-based assays.

· Poly-D (or L)-Lysine Coated Surfaces main features
Biomat's Poly-D (or L)-Lysine Coated 96-Well plates have the following characteristics:
Ready to use
Manufactured under ISO:9001 guidelines
Manufactured under aseptic conditions
Lid present on each single plate
Certificate of Quality is released for every lot
For Research Use Only

· Poly-D (or L)-Lysine Coated Surfaces Product Specifications
Coating
Poly-D (or L)-Lysine (M.W. 70-150 kDa)

Stability
The microplates, if unopened, are sterile and stable refrigerated until the expiration date printed on the label.