Name: 3-Methyladenine
Price: 180.00 USD

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    3-Methyladenine is an inhibitor of class III phosphoinositide 3-kinase (PI3K) [1].
    3-Methyladenine is widely used as an autophagy inhibitor through inhibiting class III PI3K without significantly affecting protein synthesis or ATP levels. 3-Methyladenine also inhibits class I PI3K. It is found that 3-Methyladenine has different effects on class I and class III PI3K. It blocks class I PI3K persistently while inhibits class III PI3K transiently. As an inhibitor of autophagy, 3-Methyladenine is usually used to study the role of autophagy. In addition, 3-Methyladenine is also found to have anti-cancer efficacy. It induces cell death of tumor under nutrient-starved conditions. In HT1080 cells, 3-Methyladenine significantly inhibits cell migration through reducing membrane ruffle and lamellipodia formation in normal culture condition. It also inhibits the invasion of HT1080 cells [1, 2].
    
    [1] Wu Y T, Tan H L, Shui G, et al. Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. Journal of Biological Chemistry, 2010, 285(14): 10850-10861.
    [2] Ito S, Koshikawa N, Mochizuki S, et al. 3-Methyladenine suppresses cell migration and invasion of HT1080 fibrosarcoma cells through inhibiting phosphoinositide 3-kinases independently of autophagy inhibition. International journal of oncology, 2007, 31(2): 261-268.
- Properties
  - Alternative Name
    
    3-MA; 3-methylpurin-6-amine
    
    CAS Number
    
    5142-23-4
    
    Molecular Formula
    
    C6H7N5
    
    Molecular Weight
    
    149.15
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥5 mg/mL in H2O; ≥7.45 mg/mL in DMSO; ≥8.97 mg/mL in EtOH
    
    Storage
    
    Store at -20°C
    
    * For Research Use Only
- Reference
  - 1. Du Yu, Yundi Zhao, et al. "C19orf66 Inhibits Japanese Encephalitis Virus Replication by Targeting -1 PRF and the NS3 Protein." Virol Sin. 2021 Dec;36(6):1443-1455. PMID:34309824
    2. Chenghao Li, Jiawei Li, et al. "Isorhamnetin Promotes MKN-45 Gastric Cancer Cell Apoptosis by Inhibiting PI3K-Mediated Adaptive Autophagy in a Hypoxic Environment." J Agric Food Chem. 2021 Jul 28;69(29):8130-8143. PMID:34269571
    3. Renjie Dou, Jinjun Qian, et al. "Suppression of steroid 5α-reductase type I promotes cellular apoptosis and autophagy via PI3K/Akt/mTOR pathway in multiple myeloma." Cell Death Dis. 2021 Feb 24;12(2):206. PMID:33627630
    4. Xiaoli Liu, Yan Gu, et al. "Honokiol induces paraptosis-like cell death of acute promyelocytic leukemia via mTOR & MAPK signaling pathways activation." Apoptosis. 2021 Apr;26(3-4):195-208. PMID:33550458
    5. Shichao Lv, Peng Yuan, et al. "QiShenYiQi pill improves the reparative myocardial fibrosis by regulating autophagy." J Cell Mol Med 2020 Sep 2. PMID:32881330
    6. Baicun Li, Jie Yao, et al. "Design, synthesis, and biological evaluation of 5-((8-methoxy-2-methylquinolin-4-yl)amino)-1H-indole-2-carbohydrazide derivatives as novel Nur77 modulators." Eur J Med Chem. 2020 Oct 15;204:112608. PMID:32717483
    7. Meng XL, Fu P, et al. "Increased EZH2 Levels in Anterior Cingulate Cortex Microglia Aggravate Neuropathic Pain by Inhibiting Autophagy Following Brachial Plexus Avulsion in Rats." Neurosci Bull. 2020;10.1007/s12264-020-00502-w. PMID:32346844
    8. Zhao F, Wang J, et al. "Neuroprotection by Walnut-derived Peptides through Autophagy Promotion via Akt/mTOR Signaling Pathway against Oxidative Stress in PC12 Cells." J Agric Food Chem. 2020;10.1021/acs.jafc.9b08252. PMID:32090563
    9. Zhang H, He Y, et al."miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia." Redox Biol. 2020 Jan;29:101402. PMID:31926626
    10. Zhou H, Du R, et al. "Cannabinoid receptor 2 promotes the intracellular degradation of HMGB1 via the autophagy-lysosome pathway in macrophage." Int Immunopharmacol. 2020;78:106007. PMID:31806570
    11. Chen Y, Zhu E, et al. "Important roles of C-terminal residues in degradation of capsid protein of classical swine fever virus." Virol J. 2019 Nov 6;16(1):127. PMID:31694654
    12. Ye J, Xue M, et al. "Diosbulbin B-Induced Mitochondria-Dependent Apoptosis in L-02 Hepatocytes is Regulated by Reactive Oxygen Species-Mediated Autophagy." Front Pharmacol. 2019 Jun 19;10:676. PMID:31275148
    13. Tang RH, Qi RQ, et al. "Interleukin-4 affects microglial autophagic flux." Neural Regen Res. 2019 Sep;14(9):1594-1602. PMID:31089059
    14. Sarah Needs. "The Biochemical Basis of Congenital Disorders of Glycosylation." The Open University. 2019.
    15. Gierisch ME, Pfistner F, et al. "Proteasomal Degradation of the EWS-FLI1 Fusion Protein Is Regulated by a Single Lysine Residue." J Biol Chem. 2016 Dec 23;291(52):26922-26933. PMID:27875302
    16. Xue H, Yuan G, Guo X, et al. "A Novel Tumor-promoting Mechanism of IL6 and the Therapeutic Efficacy of Tocilizumab: Hypoxia-induced IL6 Is a Potent Autophagy Initiator in Glioblastoma via the p-STAT3-MIR155-3p-CREBRF Pathway[J]." Autophagy, 10 May 2016.

3-Methyladenine

3-Methyladenine

Background

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