10x Ammonium Buffer, 15 mM MgCl2

Ammonium Buffer is recommended for most PCR applications. It results in high yield of PCR products and minimises the need for optimisation of Mg2+ concentrations or the annealing temperatures.

FEATURES
Promotes amplification on most DNA templates
High yield
High specificity
Tolerance for primer annealing temperature
10x Buffer

AVAILABLE IN 4 FORMULATIONS:
15 mM MgCl2,
Mg2+ free
Tween free
Mg2+ free + Tween free

Ammonium Buffer promotes high specificity over a broad range of annealing temperatures and Mg2+ concentrations. Ammonium Buffer also works well when dealing with difficult templates, e.g. GC-rich DNA sequences.

TIP - CHOOSE THE RIGHT BUFFER
Mg2+ free buffer is recommended if you need to optimise Mg2+ concentrations in your PCR set-up, especially if your application requires Mg2+ concentration lower than 1.5 mM.
Detergent free buffers are recommended for automation and downstream applications involving fluorescent spectrometry.

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