Name: 0.5M EDTA Buffer (pH 8.0)
Price: 285.00 USD

- Overview
  - Ethylene-diamine-tetra acetic acid (EDTA) is a high quality and convenient solution that chelates a variety of polyvalent cations such as Ca2+ and Mg 2+. EDTA is usually used as an inactivator of metal-dependent enzymes, preventing damage to DNA and RNA. In cell cultures, it is used to avoid clumping of cells in liquid suspensions, as EDTA binds to calcium and prevents joining of cadherins between cells.
    
    Pouch format:
    – Exactly pre-weighed powder (500 mL/pouch)
    Aqueous solution format:
    – 100 mL of 0. 5M EDTA Solution
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    PBS with Tween™ 20 (pH 7.6) (20x)
    
    Other Properties
    
    Chemicals: Analytical grade.
    Concentration: 0.5 M EDTA.
    pH: 8± 0.05 at 25 °C.
    
    Storage
    
    Room temperature.
    
    * This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.
- Applications
  - Application Description
    
    Anticoagulant for blood samples and its storage.
    Abduct the metal required to metal-dependent enzyme, inactivating the reactions.
    Avoid junctions between cells by cadherins, usually used to cell culture procedures.
    Used in TAE and TBE buffers because it inhibits metal-dependent nucleases by chelating the divalent cations (Ca2+ Mg2+), protecting the DNA from nucleases during the run.
    Added to TE buffer, used to solubilize DNA and RNA, inactivating nucleases by binding to metals cations required by these enzymes.

Datasheet

Documents

Brochure

0.5M EDTA Buffer (pH 8.0)

0.5M EDTA Buffer (pH 8.0)

Documents

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