Titan Taq DNA polymerase

Titan Taq DNA Polymerase is highly processive, thermostable DNA polymerase (Fig.1). The enzyme has 5’ to 3’ polymerisation-dependent exonuclease replacement activity but lacks 3’ to 5’ exonuclease activity. The enzyme has “extendase activity” allowing TA cloning.

Quality control:
The enzyme is free of nicking and priming activities, exonucleases and unspecific endonucleases. SDS/PAGE – 95 kD band, >98% pure. Activity and stability tested via thermocycling. The error rate per nucleotide per cycle is ~2.5 x 10-5; the accuracy is ~ 4 x 104. Estimated half life at 95°C is 1.5 hours.

Storage & Dilution buffer:
50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Supplied with:
10 x Reaction Buffer (Mg2+ free): 800 mM Tris-HCl pH 9.4 at 25°C, 200 mM (NH4)2SO4, 0.2% w/v Tween-20.
25 mM MgCl2

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