Titan HotTaq Probe qPCR Universal Mix

Titan HotTaq Probe qPCR Universal Mix is optimized for real-time quantitative PCR assays and contains all the components necessary to perform singleplex or duplex qPCR, with the exception of template, primers, and probes. The qPCR Mix contains optimized components and Titan HotTaq DNA polymerase supplied in a proprietary reaction buffer that enables efficient amplification of regular and GC-rich targets. Titan HotTaq Probe qPCR Universal Mix is optimized for DNA/LNA hydrolysis probes based on the 5′ flap endonuclease activity. Titan HotTaq DNA polymerase is activated by a 10 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Composition:
Titan HotTaq DNA polymerase
Titan HotTaqProbe qPCR Universal buffer
15 mM MgCl2 1x PCR solution – 3 mM MgCl2
dNTPs, including dUTP. The mix allows UNG treatment to prevent carryover contamination from previous runs.
In a separate vial: 100% DMSO is included in the kit in a separate vial.

IMPORTANT: UNG is not included in the Titan HotTaq Probe qPCR Universal Mix and must be purchased separately.

Internal reference based on ROX dye*.

For multiplex application: if ROX dye is used as one of the fluorophores, internal reference might interfere with the signal – a version without ROX is available upon request.

*ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Please contact us at for specific academic pricing.