Titan HotTaq EvaGreen® qPCR Universal Mix

Titan HotTaq EvaGreen® qPCR Universal Mix is optimized for real-time quantitative PCR assays. The ready-to-use mix includes Titan HotTaq DNA polymerase and other components necessary to perform the reaction – only water, template and primers needs to be added.

Titan HotTaq DNA polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Advantages:
Specific and reproducible real time PCR
Highly efficient in case of low copy number targets
Superior performance with long (up to 500 bp) and GC-rich templates
UNG treatment capability due to combination of dUTP/dTTP
Easy pipetting due to blue visualisation dye

Composition:
Titan HotTaq DNA polymerase
qPCR buffer
5 mM MgCl2: 1x PCR solution – 2.5 mM MgCl2
dNTPs, including dUTP/dTTP
EvaGreen® dyeInternal reference (based on ROX dye)
GC-enhancer
Visualisation dye

Please contact us at for specific academic pricing.