Titan HotTaq EvaGreen® HRM Mix (ROX)

Titan HotTaq EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: Titan HotTaq DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. Only water, template and primers need to be added.
Titan HotTaq DNA Polymerase is activated by a 12 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Titan HotTaq EvaGreen® HRM Mix is available with ROX or no ROX dye.

Composition:
Titan HotTaq DNA polymerase
5 x EvaGreen®HRM buffer
dNTPs
5 mM MgCl2 1 x PCR solution – 2.5 mM MgCl2
EvaGreen® dye*
BSA
ROX dye**
or no ROX dye

* EvaGreen® is a DNA-binding dye for qPCR that, compared to SYBR® Green I, has similar spectra but much less PCR inhibition. It is extremely stable and nonmutagenic/ noncytotoxic. EvaGreen® is compatible with all common qPCR cyclers by selecting the standard settings for SYBR® Green or FAM.
“EvaGreen® is a registered trademark of BIOTIUM, INC.”

**ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Please contact us at for specific academic pricing.