Name: T4 DNA Recombinant Protein
Price: Inquiry

- Overview
  - T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 -phosphate and 3 -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.
    
    Source
    
    Escherichia colilambda lysogen
    
    Type
    
    Recombinant Proteins
    
    Formulation
    
    50mM KCl, 10mM Tris-HCl (pH 7.4), 0.1mM EDTA, 1mM DTT, 200 µg/ml BSA and 50% glycerol.
    
    Storage
    
    50mM KCl, 10mM Tris-HCl (pH 7.4), 0.1mM EDTA, 1mM DTT, 200 µg/ml BSA and 50% glycerol. Store at -20°C.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application Description
    
    One Weiss unit is equivalent to circa 67 cohesive-end ligation units.
    T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM.
    Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzyme to achieve the same extent of ligation as cohesive-end DNA fragments. Blunt-end ligation may be enhanced by addition of PEG 4000 (10% w/v final concentration) or hexamine chloride, or by reducing the ATP concentration to 50µm.
    To dilute T4 DNA Ligase that will subsequently be stored at -20°C, 50% glycerol storage buffer should be used; to dilute for immediate use, 1x T4 DNA Ligase reaction buffer can be used.

T4 DNA Recombinant Protein

T4 DNA Recombinant Protein

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