SEPLITE®6FF Chromatographic Media

• The sample volume should be concentrated as much as possible before using the gel filtration.
• The sample must be clarified without particles.
• In order to obtain the highest resolution, the loading volume cannot exceed 5% of the bed volume.
• Column chromatography should be performed after the sample and the chromatographic medium must be thoroughly equilibrated with the elution buffer.
• The column bed must be flat, free of grooves and air bubbles, otherwise it should be reassembled.
• In order to protect the stability and activity of the protein, a buffer purification protein is selected.
• In order to avoid non-specific ionic interaction between protein and medium, up to 0.15 mol/L NaCl can be added to the buffer.
• According to the molecular weight of the target protein, the medium closest to the median separation range is selected.
• The flow rate should be strictly controlled during the elution process, and not too fast.
• During the loading and the entire elution process, the cylinder surface is prevented from drying out.
• This product should avoid contact with oxidizing agents and avoid prolonged exposure to the air.

Agarose Chromatography Resin