Recombinant Mouse Bone Sialoprotein 2/IBSP (C-6His)

Recombinant Mouse Bone Sialoprotein 2/IBSP (C-6His)

Catalog Number:
P001411155ABE
Mfr. No.:
Abe32-7833
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      • Overview
        • MW : 35.1kD. Recombinant Mouse Bone Sialoprotein 2 is produced by our Mammalian expression system and the target gene encoding Phe17-Gln324 is expressed with a 6His tag at the C-terminus. IBSP, is a monomeric non-collagenous member of the SIBLING family of extracellular matrix proteins. It is principally associated with the early stages of bone mineralization. Mouse IBSP is synthesized as a 324 amino acid (aa) precursor that contains a 16 aa signal sequence and a 308 aa mature region. The mature segment is divided into a basic N-terminus (aa 17 - 62), a central region (aa 63 - 233), and an acidic C-terminus (aa 234 - 317). IBSP is highly glycosylated, sulfated and phosphorylated. Phosphorylation promotes HAp nucleation, while carbohydrate may regulate cell adhesion.

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      • Properties
        • Source
          Human cells
          Type
          Recombinant Proteins
          Formulation
          Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
          Storage
          Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
          Endotoxin
          Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

          More Information

          UniProt
          Gene ID
          15891

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

      • Applications
        • Application Description
          Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

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