Name: Recombinant Human Vanin
Price: Inquiry

- Overview
  - VNN1 Human Recombinant produced in E. Coli is a single, non-glycosylated, polypeptide chain (Gln22-Gly491) containing 480 amino acids including a 6 aa His tag at C-terminus. The total predicted molecular mass is 53.5kDa, but it migrates as an approximately 70-80kDa. Vanin 1 (VNN1) belongs to the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. This family includes secreted and membrane-associated proteins, a few of which have been described to participate in hematopoietic cell trafficking. No biotinidase activity has been established for any of the vanin proteins; nevertheless, they possess pantetheinase activity, which may have a role in oxidative-stress response. VNN1 protein, like its mouse homolog, is probably a GPI-anchored cell surface molecule. The mouse VNN1 protein is expressed by the perivascular thymic stromal cells and regulates migration of T-cell progenitors to the thymus. VNN1 is an amidohydrolase which hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine.
    
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- Properties
  - Protein Name
    
    Vanin 1, Vascular Non-Inflammatory Molecule 1, Pantetheine Hydrolase, EC 3.5.1.92, Vanin-1, HDLCQ8, Tiff66, Pantetheinase, Vannin 1, EC 3.5.1, VNN1.
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 95% as determined by reduced SDS-PAGE.
    
    Formulation
    
    VNN1 was filtered (0.2µm) and lyophilized in 20mM PB buffer, 150mM NaCl pH 7.2.
    
    Storage
    
    Store lyophilized protein at <-20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time (2-7 days); it does not show any change after two weeks at 4°C. Aliquotes of reconstituted samples are stable at <-20°C for 3 months.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application Description
    
    Always centrifuge tube before opening. DO NOT mix by vortexing or pipetting. It is not recommended to reconstitute to a concentration less than 0.1mg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Recombinant Human Vanin

Recombinant Human Vanin

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