Name: Recombinant Human Tumor Necrosis Factor a/TNFa (C-6His)
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- Overview
  - MW : 18.5kD. Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed with a 6His tag at the C-terminus. Tumor Necrosis Factor-a (TNF-a) is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells following stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-a while cells that express CD8 secrete little or no TNF-a. Synthesis of TNF-a can be induced by many different stimuli including interferons, IL2, and GM-CSF. The clinical use of the potent anti-tumor activity of TNF-a has been limited by the proinflammatory side effects such as fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis, and hemorrhage. Designing clinically applicable TNF-a mutants with low systemic toxicity has been of intense pharmacological interest. Human TNF-a that binds to murine TNF-R55 but not murine TNF-R7, exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-a, which binds to both murine TNF receptors. Based on these results, many TNF-a mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro and have exhibited lower systemic toxicity in vivo. Recombinant Human TNF-a High Active Mutant differs from the wild-type by amino acid subsitution of amino acids 1-7 with Arg8, Lys9, Arg10 and Phe157. This mutant form has been shown to have increased activity with less inflammatory side effects in vivo.
    
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- Properties
  - Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Formulation
    
    Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
    
    Storage
    
    Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
    
    Endotoxin
    
    Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
    
    More Information
    
    UniProt
    
    P01375
    
    Gene ID
    
    7124
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Recombinant Human Tumor Necrosis Factor a/TNFa (C-6His)

Recombinant Human Tumor Necrosis Factor a/TNFa (C-6His)

More Information

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