Recombinant Human Ephrin-B1/EFNB1 (C-6His)

Recombinant Human Ephrin-B1/EFNB1 (C-6His)

Catalog Number:
P001411091ABE
Mfr. No.:
Abe32-7892
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      • Overview
        • MW : 23.4kD. Recombinant Human Ephrin-B1 is produced by our Mammalian expression system and the target gene encoding Leu28-Gly232 is expressed with a 6His tag at the C-terminus. Ephrin-B1, also named EFL-3, ELK ligand, EPH-related receptor tyrosine kinase ligand 2, is a single-pass type I membrane protein. It contains 1 ephrin RBD (ephrin receptor-binding) domain and belongs to the ephrin family. Ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. All ephrins share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. Ephrin-B1 has been shown to bind EphA3, EphB1, EphB2, EphB3, and EphB4. The extracellular domains of human and mouse ephrin-B1 share 94% amino acid identity.

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      • Properties
        • Source
          Human cells
          Type
          Recombinant Proteins
          Formulation
          Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH7.4.
          Storage
          Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
          Endotoxin
          Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

          More Information

          UniProt
          Gene ID
          1947

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

      • Applications
        • Application Description
          Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

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