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Recombinant HIV-1 Protease

Recombinant HIV-1 Protease

Catalog Number:
P001414763ABE
Mfr. No.:
Abe32-5585
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  • Size:
    10 µg
    Quantity:
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      • Overview

        • HIV-1 protease is an active homodimer having a molecular mass of 21.6kDa (each monomer of 99 amino acids is 10.8kDa). HIV-1 protease is very significant in the life cycle of the HIV virus. It is expressed in the infected cells as a part of Gag-Pol polyprotein from which it is auto-catalytycaly released after formation of an immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins resulting in the maturation of the virus. In HIV-infected patients the enzyme is subjected to intensive mutagenesis and mutants resistant to applied medicines are produced as a result of the selection pressure.

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      • Properties
        • Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 95% as determined by densitometric image analysis.
          Formulation
          The HIV-1 Protease filtered (0.4µm) solution (0.27mg/1ml) is formulated in 20mM Tris, 20mM MES, 200mM NaCl, 1mM EDTA, 10% (v/v) glycerol and 0.05% 2-mercaptoethanol, pH 6.5.
          Storage
          Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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