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Recombinant Hepatitis C Virus NS5A

Recombinant Hepatitis C Virus NS5A

Catalog Number:
P001414682ABE
Mfr. No.:
Abe32-5479
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  • Size:
    10 µg
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      • Overview

        • Recombinant HCV NS5A produced in E. coli is a single polypeptide chain containing 118 amino acids (aa 2210-2306) and having a molecular mass of 13.4kDa. Recombinant HCV NS5A is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. NS5A is a component of the replication complex which takes part in RNA-binding. NS5A proteins hyperphosphorylated form is an inhibitor of viral replication. NS5A interacts with Human VAPB and through it targets the viral replication complex to vesicles. NS5A down-regulates viral IRES translation initiation and mediates interferon resistance by interacting and inhibiting human EIF2AK2/PKR. NS5a is also inhibits apoptosis by interacting with BIN1 and FKBP8.

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      • Properties
        • Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 90% as determined by SDS-PAGE.
          Formulation
          The HCV NS5A protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol and 1mM DTT.
          Storage
          Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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