Name: Recombinant Aggrecan Interglobular Domain, 100µg
Price: 878.00 USD

- Overview
  - The recombinant protein contains cleavage sites for aggrecanases (E373 - A374 in human aggrecan) and matrix metalloproteinases (N341 - F342 in human aggrecan). Substrate for aggrecanases and matrix metalloproteinases activity measurements.
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    More than 80% of total protein
    
    Formulation
    
    The protein is solubilized in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM CaCl₂.
    
    Storage
    
    Aggrecan interglobular domain is stable until the expiry date given on the label when stored at -70 °C. The protein can be kept at -20 °C for several weeks and on ice for several days. Repeated freezing and thawing should be avoided.
    
    Molecular Weight
    
    15.5 kDa
    
    * For Research Use Only
- Applications
  - Application Description
    
    Aggrecan interglobular domain is used as substrate for aggrecanases and matrix metalloproteinases. For proteinase activity measurements, the protein is incubated with proteinase for various time intervals. Thereafter, aliquots of the incubation mixture are analyzed by SDS-PAGE or by ELISA. Upon cleavage with aggrecanases the apparent Mr of aggrecan interglobular domain in SDS-PAGE is reduced from 21 kDa to about 13 kDa. Quantitative measurement of aggrecanase cleavage requires a neoepitop antibody with specificity for the N-terminus A R G S V I L T . . . appearing upon hydrolysis. The fragment with the newly formed N-terminus is fixed by the neoepitop antibody to a microplate and quantified with an anti-His-tag antibody. In analogy, cleavage by matrix metalloproteinases can be measured with antibodies to neoepitopes appearing upon action of these enzymes.

Datasheet

Recombinant Aggrecan Interglobular Domain, 100µg

Recombinant Aggrecan Interglobular Domain, 100µg

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