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Procathepsin K

Procathepsin K

Catalog Number:

P001373813BIO

Mfr. No.:

30701002; 30701003

Price:

$353

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- Overview
  - Recombinant human procathepsin K of amino acids Glu19 - Met215 is expressed in E. coli with an additional M-residue at the N-terminus. Characterization of structure and function; Screening of inhibitors; Standard in enzymatic and immunologiacal assays; Drug target by excessive bone resorption, such as osteoporosis.
    
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- Properties
  - Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    More than 95% of total protein
    
    Formulation
    
    The proenzyme is dissolved in 500 mM NaCl, 25 mM Tris-HCl, pH 8.0.
    
    Storage
    
    Procathepsin K is stable until the expiry date given on the label if stored at -80 °C. Repeated freezing and thawing must be avoided. After activation the enzyme is rather unstable. If the activated enzyme is not used immediately, it is recommended to add MMTS (1 mM final concentration). For activity measurements L-cysteine must then be added to restore the active conformation of the enzyme.
    
    * For Research Use Only
- Applications
  - Application Description
    
    1. Characterization of cathepsin K structure and function
    2. Screening of inhibitors for cathepsin K
    3. Drug target for diseases characterized by excessive bone reabsorption, such as osteoporosis

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