Name: PPP1CA Recombinant Protein
Price: Inquiry

- Overview
  - PPP1CA Human Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 350 amino acids (1-330 a.a) and having a molecular mass of 39.7kDa. PPP1CA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Serine/Threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) is crucial for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. The PPP1CA protein may have a significant role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.
    
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- Properties
  - Protein Name
    
    Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PPP1CA, PPP1A, PP1alpha.
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 80.0% as determined by SDS-PAGE.
    
    Formulation
    
    PPP1CA protein solution (0.2mg/ml) containing 50mM Tris-HCl (pH8.5), 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2 and 50% glycerol.
    
    Storage
    
    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application Description
    
    Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.

PPP1CA Recombinant Protein

PPP1CA Recombinant Protein

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