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Overview
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Background
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. POLR2I (polymerase (RNA) II (DNA directed) polypeptide I), also known as RPB9 or hRPB14.5, is a 125 amino acid nuclear protein belonging to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. Component of RNA polymerase II, POLR2I catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR2I is part of the upper jaw surrounding the central large cleft and is thought to grab the incoming DNA template.
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