OxPL Lipoprotein Assay

Assay Principle
The DZ-Lite OxPL Lipoprotein Assay is a magnetic particle based chemiluminescent immunoassay similar to the chemiluminescent ELISA assay as described by Tsimikas S. et al26-27 with modifications in Kiechl et al28 and Bertoia et al.4 In the assay, magnetic particles coated with a monoclonal antibody MB47 that specifically binds to apoB-100 of all apoB-100-containing lipoproteins (VLDL, IDL, LDL, Lp(a)) are mixed with serum samples to capture the apoB containing lipoprotein molecules to a saturation condition per magnetic particle. After a washing step to remove unbound apoB-100 molecules, the detecting antibody, ABEI-labeled E06, is added to the reaction mixture to allow the E06 monoclonal antibody to react with OxPL on the captured apoB-100 molecules. After another wash to remove the unbound E06, the reaction mixture is added to a trigger reagent to generate a chemiluminescent signal (relative light units, RLU) measured by a photomultiplier. The amount of OxPL Lipoprotein is quantified by using a 10-point calibration curve. The results are reported as nM OxPL Lipoprotein. The assay is fully automated with a DZ-Lite chemiluminescent immunoanalyzer.

Features
· Specific for oxidized phosphocholines on apoB-100 containing lipoproteins
· Assay is fully automated
· No manual sample pre-dilution required
· A substantial amount of clinical data strongly supports the link between OxPL-apoB level and risks of CVD

Please contact us at for specific academic pricing.