OptiPrep™ Cell Separation Media

OptiPrep™ Cell Separation Media

Catalog Number:
CS02528369PRO
Mfr. No.:
PRO-1114542
Price:
$416
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      • Overview
        • OptiPrep™ is a multi-purpose, sterile and endotoxin tested density gradient medium based on non-ionic iodixanol.
          It has significantly lower toxicity compared to ionic gradient compounds such as metrizamide.
          This density gradient medium can be used to isolate and purify DNA, virus RNA, cell organelles and a wide variety of cell types.
          Cell separation is made easier with OptiPrep™ due to its high density.
          With OptiPrep™, cell flotation on a dense loading zone can be achieved either through a continuous or a discontinuous gradient or through a basic density barrier.
          It can be used clinically in X-ray contrast imaging.

          Advantages of OptiPrep™ vs Other gradients:
          • OptiPrep™ vs Histopaque & Percoll
          Percoll and Histopaque include one or several washing step(s) prior to their use. The removal step(s) is/are required to avoid the formation of density gradients which can be toxic to mammalian cells. Unlike Histopaque and Percoll, OptiPrep™ has no potential for bioaccumulation, therefore is non-toxic for the cell. OptiPrep™ has a better yield, purity and viability (92%, 86%, 54%) compared to Histopaque (58%, 61%, 54%).
          • OptiPrep™ vs CsCl (Caesium chloride)
          Particle virus number ratios are 100 times higher with OptiPrep™ compared to those recorded from CsCl gradients.

          OptiPrep™ can be a good substitution of:
          • Histopaque
          • Percoll
          • Cesium Chloride
          • Iodixanol
          • 92339-11-2
          • Iodixanolum
          • Indixanol
          • Iodixanolum [Latin]
          • UNII-HW8W27HTXX
          • CCRIS 7567
          • HW8W27HTXX
          • CHEBI:31705
          • 2-5410-3A
          • 5,5'-((2-Hydroxytrimethylene)bis(acetylimino))bis(N,N'-bis(2,3-dihydroxypropyl)-2,4,6-triiodoisophthalamide)
          • DSSTox_CID_25523
          • DSSTox_RID_80929
          • DSSTox_GSID_45523
          • 1,3-Benzenedicarboxamide, 5,5'-((2-hydroxy-1,3 propanediyl)bis(acetylimino))bis(N,N'-bis(2,3-dihydroxypropyl)-2,4,6-triiodo-
          • Iodixanol [USAN:BAN:INN]

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Cell separation media
          Other Properties
          Iodixanol: 60% (w/v) in water
          Storage
          OptiPrep™ Should be stored between 4°C - 24°C in a sterile and dark environment.
          It can be stored up to 3 years.
          Avoid long exposures to sunlight ( NB. The effect of sunlight exposure is negligible when working with OptiPrep™ on a day to day basis).
          Before use invert the bottles several times to mix the contents.
          Shipping
          Room temperature, large volume

          * For Research Use Only.

      • Applications
        • Application Description
          Cell Isolation, Isolation of subcellular organelles and membranes, Virus purification, Macromolecules and lipoproteins purification
      • Reference

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