Novo-Light® 4-color Multiplex Fluorescence Immunohistochemistry Kit

Multiple fluorescent immunohistochemical staining is based on the principle of specific binding of antigen and antibody. Horseradish peroxidase (HRP)-labeled secondary antibodies are used to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. With the help of different fluorescent dye labels, multiple rounds of staining cycles can achieve in situ multi-target staining of tissues or cells.

Detection principle
Tyramine signal amplification technology principle: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibodies. HRP catalyzes the addition of fluorescent substrates to the system to produce activated fluorescent substrates. The activated substrates can covalently bind to tyrosine on the antigen, making the sample stably covalently bound to fluorescent. After that, the non-covalently bound antibodies are washed away by heat repair, and then a primary antibody is replaced for the second round of incubation, and another fluorescent substrate is replaced. Multiple labeling can be achieved by repeating this process.

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