NL5 Fast & Deep 3D Confocal Microscope

Spinning disk systems are great but when going deeper they all show pinhole crossover issues causing image quality degradation. Also they need "help" to create the uniformity of illumination.

NL5 is using slit apertures, rather than pinholes, to construct a confocal imaging system, which has some advantages. The signal level is increased and, if a detector array is used, a line image can be generated in real time. Slit apertures can also be used in a direct-view confocal (tandem scanning) microscope.

Convert your existing research microscope into fast scanning confocal system ideal for deep 3D live cell imaging with NL5.
Brief Introduction
NL5 is a fast line-scanning confocal system with high sensitivity and resolution. It allows you to quickly screen a multi-well plate with multicolor images, from which you can select the most promising ones. Capture 3D live samples with minimal phototoxicity, high resolution and contrast. Like RCM, NL5 keeps your samples happy under the microscope for a long time and is easy to use. Convert your research microscope into a fast-scanning confocal microscope that provides very gentle conditions for your live samples.

The Benefits of NL5
· Single Digital scanner (same type as RCM2)
· Faster scanning speed than RCM2
· High contrast deep 3D imaging without pinhole cross-talk
· Better resolution for deep 3D imaging than spinning disk
· 16x brighter than spinning disk
· High sensitivity and resolution (170 nm after deconvolution)
· Hardware integration into third-party software
· Extreme sensitivity ensures low phototoxicity, live cell friendly
· Compact & easy to use
· Fast scanning speed, low levels of phototoxicity:

In the field of biology, some natural processes happen fast, like cell and protein dynamics and cell-cell interactions. Therefore, fast scanning speed is needed to study them. And that is exactly what NL5 brings to the table! In addition, researchers in developmental biology, regeneration or immunology fields often need to image large live samples (mouse embryos, zebrafish, organoids) for a long time without losing any detail of the cell's behavior. In that case, a combination of speed and a low phototoxicity system like NL5 is perfect.

The benefits of using NL5:
· NL5 is a fast scanning confocal system that reaches 55 fps (full frame), much faster than regular point-scanning confocal systems. In addition to speed, it brings very gentle imaging conditions for your samples as well. Perform live cell imaging for more than a day, with no signs of phototoxicity. NL5 is easy to use, and requires basic training to operate.
· NL5 does not cause any pinhole cross-talk, as it has only one slit pinhole. Pinhole cross-talk is associated with spinning disk systems, because they contain a disk with multiple pinholes combined and the out-of-focus light can sometimes pass through the closeby pinholes. This effect is more evident when imaging deep within the sample, making NL5 the ideal system for those applications.
· NL5 is a cost-effective solution that can be coupled to any research microscope via the c-mount interface.

Please contact us at for specific academic pricing.