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Figure 1. Immunohistochemical identification of Tachykinin Receptor 1 in pancreatic carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NK1 (Tachykinin Receptor 1) antibody (7TM0360N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, NK1 receptors were uniformly detected at the plasma membrane of chief cell in the pancreatic carcinoma.

Figure 2. Immunohistochemical identification of Tachykinin Receptor 1 in glioblastoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NK1 (Tachykinin Receptor 1) antibody (7TM0360N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, NK1 receptors were detected predominantly at the level of plasma membrane in glioblastoma.

Figure 3. Immunohistochemical identification of Tachykinin Receptor 1 in ovarian carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NK1 (Tachykinin receptor 1) antibody (7TM0360N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, NK1 receptors were detected predominantly at the level of plasma membrane in ovarian carcinoma.

Figure 4. Validation of the Tachykinin Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Tachykinin Receptor 1 (NK1) were lysed and immunoblotted with the anti-NK1 antibody (7TM0360N-IC) at a dilution of 1:1000.

Figure 5. Immunocytochemical identification of Tachykinin Receptor 1 in HEK293 cells. HEK293 cells stably expressing the Tachykinin receptor 1 (NK1) were either not exposed or exposed to 1 μM Substance P for 30 min and immunocytochemically stained with anti-NK1 (non-phospho-Tachykinin receptor 1) antibody (7TM0360N-IC) at a dilution of 1:200. Note, NK1 receptors were confined to the plasma membrane in untreated cells (0 min). NK1 receptors were seen in perinuclear clusters of vesicles after 30 min Substance P exposure.

NK1 ( IHC-grade ), Tachykinin Receptor 1 Receptor Polyclonal Antibody

NK1 ( IHC-grade ), Tachykinin Receptor 1 Receptor Polyclonal Antibody

Catalog Number:

A001456013TMA

Mfr. No.:

7TM0360N-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The NK1 antibody is directed against the distal end of the carboxyl-terminal tail of human Tachykinin Receptor 1 (also called Substance P Receptor). It detects selectively the canonical full-length NK1 Tachykinin Receptor. It can be used to detect total NK1 receptors in Western blots independent of phosphorylation. The NK1 antibody can also be used to isolate and enrich NK1 receptors from brain lysates. It also detects NK1 in cultured cells and tissue sections by immunohistochemistry.
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  human
  
  Immunogen
  
  A synthetic peptide with sequence SSRSDSKTMTESFSFSSNVLS corresponds to amino acids 387-407 of human NK1.
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  NK1, SPR, TACR1, Neurokinin Receptor 1, Substance P Receptor (UniProt:P25103)
  
  * This product is intended for research use only.

Applications

Application

WB; ICC; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100

Reference
- Schulz S, Stumm R, Röcken C, Mawrin C, Schulz S. Immunolocalization of full-length NK1 tachykinin receptors in human tumors. J Histochem Cytochem. 2006 Sep;54(9):1015-20. Epub 2006 May 1. PubMed PMID: 16651388.

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