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Figure 1. Immunohistochemical identification of Somatostatin Receptor 3 in neuronal cilia. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST3 (Somatostatin Receptor 3) antibody (7TM0357N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, SST3 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 2. Immunohistochemical identification of Somatostatin receptor 3 in mouse brain in vivo. Brain sections from SST3-deficient mice (SST3-KO) and their wild-type littermates (SST3-WT) were dewaxed, microwaved in citric acid, and incubated with anti-SST3 (Somatostatin Receptor 3) antibody (7TM0357N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, SST3 receptors were uniformly detected at the plasma membrane of small rod-shaped cells which represent neuronal cilia.

Figure 3. Immunocytochemical identification of Somatostatin Receptor 3 in transfected HEK293 cells. HEK293 cells stably expressing the mouse Somatostatin Receptor (SST3) were either not exposed or exposed to 1 μM SRIF (somatotropin release inhibiting factor) for 30 min and immunocytochemically stained with anti-SST3 (Somatostatin Receptor 3) antibody (7TM0357N-IC) at a dilution of 1:200. Note, SST3 receptors were confined to the plasma membrane in untreated cells (0 min). SST3 receptors were seen in perinuclear clusters of vesicles after 30 min SRIF exposure.

Figure 4. Validation of the Somatostatin Receptor 3 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the mouse Somatostatin Receptor 3 receptor (SST3) were lysed and immunoblotted with the anti-SST3 antibody (7TM0357N-IC) at a dilution of 1:1000.

mSST3 ( IHC-grade ), Somatostatin Receptor 3 Polyclonal Antibody ( Mouse )

mSST3 ( IHC-grade ), Somatostatin Receptor 3 Polyclonal Antibody ( Mouse )

Catalog Number:

A001456015TMA

Mfr. No.:

7TM0357MN-IC

Price:

$528

Size:

100 µl

Quantity:

Add to Cart:

Overview
- The mouse SST3 antibody is directed against the distal end of the carboxyl-terminal tail of mouse and rat Somatostatin Receptor 3. It can be used to detect total SST3 receptors in Western blots independent of phosphorylation. The SST3 antibody can also be used to isolate and enrich SST3 receptors from tissue lysates. It also detects SST3 in cultured cells and tissue sections by immunohistochemistry. The SST3 antibody has been validated using knockout mice (KO-Validated).
  
  Please contact us at for specific academic pricing.
Properties
- Host
  
  rabbit
  
  Antibody Type
  
  polyclonal
  
  Isotype
  
  IgG
  
  Reactivity
  
  mouse; rat
  
  Immunogen
  
  A synthetic peptide presents distal end of carboxyl-terminal tail of mouse and rat SST3
  
  Purification
  
  Antigen affinity chromatography
  
  Formulation
  
  Liquid
  
  Storage
  
  Short-term storage at 4°C and long-term storage at -20°C
  
  Antigen
  
  SST3, SSTR3, Somatostatin Receptor 3 (UniProt:P30935 (mouse), P30936 (rat))
  
  * This product is intended for research use only.

Applications

Application

WB; ICC; IHC

Application	Dilutions
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100

Reference
- Händel M, Schulz S, Stanarius A, Schreff M, Erdtmann-Vourliotis M, Schmidt H, Wolf G, Höllt V. Selective targeting of somatostatin receptor 3 to neuronal cilia. Neuroscience. 1999 Mar;89(3):909-26.
  Pfeiffer M, Koch T, Schröder H, Klutzny M, Kirscht S, Kreienkamp HJ, Höllt V, Schulz S. Homo- and heterodimerization of somatostatin receptor subtypes. Inactivation of sst(3) receptor function by heterodimerization with sst(2A). J Biol Chem. 2001 Apr 27;276(17):14027-36. Epub 2000 Dec 27.
  Einstein EB, Patterson CA, Hon BJ, Regan KA, Reddi J, Melnikoff DE, Mateer MJ, Schulz S, Johnson BN, Tallent MK. Somatostatin signaling in neuronal cilia is critical for object recognition memory. J Neurosci. 2010 Mar 24;30(12):4306-14. doi: 10.1523/JNEUROSCI.5295-09.2010. PubMed PMID: 20335466; PubMed Central PMCID: PMC3842454.

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